FINE STRUCTURES OF INTERPHASE NUCLEI

Fine Structural Alterations of Interphase Nuclei of Lymphocytes Stimulated to Growth Activity in Vitro

This report describes fine structural changes of interphase nuclei of human peripheral blood lymphocytes stimulated to growth by short-term culture with phytohemagglutinin. Chromatin is found highly labile, its changes accompanying the sequential increases of RNA and DNA synthesis which are known to occur in lymphocyte cultures. In "resting" lymphocytes, abundant condensed chromatin appears as ...

Chromatin associations in Arabidopsis interphase nuclei

The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analyzed Arab...

Heterochromatic Patterns in Drosophila virilis Interphase Nuclei

Fluorescent dot counting in interphase cell nuclei

Fluorescence in situ hybridization allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analysed, particularly when the frequency of aberrant cells is low. Automation of dot counting is desirable because manual counting is tedious, fatiguing, an...

Asynchronous Dna Synthesis of Sex Chromatin in Human Interphase Nuclei

The sex chromatin body is an oval or round chromatin mass approximately 1 micron in diameter lying against the inner portion of the nuclear membrane. In human tissue it is present in about 20 to 40 per cent of female cells and is absent in male cells. The sex chromatin of somatic interphase nuclei is thought to be derived from a portion of a single heteropyknotic X chromosome (1, 2). Lima-de-Fa...